Beer Lambert Law Calculator
Beer-Lambert Law Calculator
The Beer Lambert Law Calculator, also known as the Beer-Lambert-Bouguer Law, is a fundamental principle in analytical chemistry and spectroscopy. It provides a relationship between the concentration of a substance in a solution, the amount of light it absorbs (measured as absorbance), the molar absorptivity (ε), and the path length (b) through which the light travels in the solution. This law is essential for quantifying the concentration of a solute in a sample when the other parameters are known.
Calculate the concentration of a substance using the Beer-Lambert Law.
Concentration (M):
Understanding the Beer-Lambert Law
The Beer-Lambert Law can be expressed as:
Absorbance (A) = ε * c * b
Where:
- Absorbance (A): A dimensionless measure of how much light is absorbed by a solution at a specific wavelength. It is often denoted as “A.”
- Molar Absorptivity (ε): A constant characteristic of the substance and the wavelength of light used in the measurement. It is a measure of how strongly a substance absorbs light.
- Concentration (c): The amount of the substance (in moles) per unit volume of the solution. It is expressed in molarity (M).
- Path Length (b): The distance that light travels through the solution, typically measured in centimeters (cm).
The Beer-Lambert Law Calculator
The Beer-Lambert Law Calculator simplifies the process of determining the concentration of a substance in a solution using the Beer-Lambert Law. To calculate the concentration, follow these steps:
Absorbance (A): Enter the absorbance value (A) obtained from the spectrophotometer or measurement equipment into the “Absorbance (A)” field.
Molar Absorptivity (ε): Input the molar absorptivity (ε) of the substance and the wavelength of light used in the measurement. This value is specific to the substance and the conditions of the measurement.
Path Length (b, cm): Specify the path length (b) through which the light travels in the solution. This is usually measured in centimeters (cm).
Click the “Calculate Concentration” button.
The calculator will apply the Beer-Lambert Law formula and display the concentration of the substance in moles per liter (M). The result provides valuable information for various scientific and analytical applications, such as determining the concentration of a solute in a chemical solution.
In summary, the Beer-Lambert Law Calculator is a valuable tool for chemists, researchers, and analysts working in fields such as chemistry, biology, and environmental science. By simplifying the calculation process and ensuring accurate results, it contributes to the precise determination of substance concentrations in various samples and solutions.